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Uracil DNA Glycosylase

peqGOLD Uracil DNA-Glycosylase (UDG) in combination with dUTP prevents contaminations in PCR reactions.

After incorporation of uracil into the DNA strand, UDG catalyses the hydrolysis of the n-glycosidic bond between uracil and deoxyribose. Before starting the PCR an incubation at 37 °C for 10 minutes degrades uracil containing DNA fragments from previous experiments. The enzyme is deactivated by heat shock at 95 °C for 10 minutes.

Total 2 products |  Page(s)   1 

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Total 2 products |  Page(s)   1 
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uracil dutp udg peqgold uracil dna-glycosylase contamination pcr
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